Journal: PLOS ONE

Article Title: Rosiglitazone alleviates LPS-induced endometritis via suppression of TLR4-mediated NF-κB activation

doi: 10.1371/journal.pone.0280372

Figure Lengend Snippet: Primers of human IL-1β, IL-6, TLR4 and β-actin.

Article Snippet: Rosiglitazone (BRL-49653) and a TLR4 signaling inhibitor TAK-242 (CLI-095) were purchased from MedChemExpress (NJ, USA) and dissolved in dimethyl sulfoxide (DMSO).

Techniques:

Journal: PLOS ONE

Article Title: Rosiglitazone alleviates LPS-induced endometritis via suppression of TLR4-mediated NF-κB activation

doi: 10.1371/journal.pone.0280372

Figure Lengend Snippet: a DMSO control group, b RGZ control group, c LPS group, d LPS + RGZ group. TLR4 staining levels in endometrial tissues showed a reduced intensity in LPS + RGZ group compared to the LPS group. TLR4 staining levels in endometrial tissue indicated a reduced intensity in the RGZ group compared to the DMSO control group. The values presented were the means ± SEM (n = 10). ns, not significant, # P < 0.05 and * P < 0.05 compared with DMSO control group and LPS group, respectively.

Article Snippet: Rosiglitazone (BRL-49653) and a TLR4 signaling inhibitor TAK-242 (CLI-095) were purchased from MedChemExpress (NJ, USA) and dissolved in dimethyl sulfoxide (DMSO).

Techniques: Control, Staining

Journal: PLOS ONE

Article Title: Rosiglitazone alleviates LPS-induced endometritis via suppression of TLR4-mediated NF-κB activation

doi: 10.1371/journal.pone.0280372

Figure Lengend Snippet: The protein levels of TLR4, p-p65 and p-IκBα in uterus tissues were measured by Western blotting. Data were represented the mean ± SEM (n = 10). # P < 0.05 and * P < 0.05 compared with DMSO control group and LPS group, respectively.

Article Snippet: Rosiglitazone (BRL-49653) and a TLR4 signaling inhibitor TAK-242 (CLI-095) were purchased from MedChemExpress (NJ, USA) and dissolved in dimethyl sulfoxide (DMSO).

Techniques: Western Blot, Control

Journal: PLOS ONE

Article Title: Rosiglitazone alleviates LPS-induced endometritis via suppression of TLR4-mediated NF-κB activation

doi: 10.1371/journal.pone.0280372

Figure Lengend Snippet: a-b mRNA levels of IL-1β and IL-6 in LPS-stimulated HESCs. GAPDH was used as a control. Control is Effects of RGZ on cytokines and TLR4 expression. the control group; LPS (0.01,0.05,0.25,1,5μg/mL) are the LPS groups. c-e Control is the control group; LPS (1μg/mL) is the LPS group; and 10 and 20 are the RGZ treatment groups representing 10 μM/L and 20 μM/L per cell plate, respectively. The data presented were the means ± SEM. ## P < 0.01, * P < 0.05 and ** P < 0.01 compared with control group and LPS group, respectively.

Article Snippet: Rosiglitazone (BRL-49653) and a TLR4 signaling inhibitor TAK-242 (CLI-095) were purchased from MedChemExpress (NJ, USA) and dissolved in dimethyl sulfoxide (DMSO).

Techniques: Control, Expressing

Journal: PLOS ONE

Article Title: Rosiglitazone alleviates LPS-induced endometritis via suppression of TLR4-mediated NF-κB activation

doi: 10.1371/journal.pone.0280372

Figure Lengend Snippet: The protein levels of TLR4, p-p65 and p-IκBα in HESCs were measured by Western blotting. GAPDH served as the control. Control is the control group; LPS is the LPS group; TAK-242 was the 1 μM/L TAK-242 treatment groups per cell plate, RGZ was the 10 μM/L RGZ treatment groups per cell plate, LPS+RGZ was the 30min incubation of the cell pretreatment with 10 μM RGZ followed by LPS induced for 24 h incubation, LPS+RGZ+TAK-242 was the 2 h incubation of 1 μM TAK-242 combined with 30min incubation of with 10 μM RGZ followed by LPS induced for 24 h incubation. Data represent the mean ± SEM. ## P < 0.01, * P < 0.05 and ** P < 0.01 compared with control group and LPS group, respectively.

Article Snippet: Rosiglitazone (BRL-49653) and a TLR4 signaling inhibitor TAK-242 (CLI-095) were purchased from MedChemExpress (NJ, USA) and dissolved in dimethyl sulfoxide (DMSO).

Techniques: Western Blot, Control, Incubation

Journal: PLOS ONE

Article Title: Rosiglitazone alleviates LPS-induced endometritis via suppression of TLR4-mediated NF-κB activation

doi: 10.1371/journal.pone.0280372

Figure Lengend Snippet: Translocation of the NF-κB p65 subunit from the cytoplasm into the nucleus was assessed by immunofluorescence staining. After LPS stimulation, TLR4 was markedly increased in uterus tissue compared to the control group. Treatment with RGZ and RGZ+TAK-242 reduced the expression of TLR4. Scale bar: 50 μm. Blue spots represent cell nuclei, and green spots indicate TLR4 staining. red spots indicate p-p65 staining.

Article Snippet: Rosiglitazone (BRL-49653) and a TLR4 signaling inhibitor TAK-242 (CLI-095) were purchased from MedChemExpress (NJ, USA) and dissolved in dimethyl sulfoxide (DMSO).

Techniques: Translocation Assay, Immunofluorescence, Staining, Control, Expressing